CRISPR/Cas9 as Tool for Functional Study of Genes Involved in Preimplantation Embryo Development

Abstract

The CRISPR/Cas9 system has proven to be an efficient gene-editing tool for genome modification of cells and organisms. However, the applicability and efficiency of this system in pig embryos have not been studied in depth. Here, we aimed to remove porcine OCT4 function as a model case using the CRISPR/Cas9 system. Injection of Cas9 and single-guide RNA… (More)
DOI: 10.1371/journal.pone.0120501

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