CRISPR/Cas9 and TALEN-mediated knock-in approaches in zebrafish.

@article{Auer2014CRISPRCas9AT,
  title={CRISPR/Cas9 and TALEN-mediated knock-in approaches in zebrafish.},
  author={Thomas Oliver Auer and Filippo Del Bene},
  journal={Methods},
  year={2014},
  volume={69 2},
  pages={142-50}
}
The targeted introduction of mutations utilizing sequence specific transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system (RNA-guided nucleases, RGNs) has revolutionized reverse genetic approaches in numerous model organisms. In zebrafish, both systems were successfully applied to generate loss-of-function alleles by targeting open reading frames or deletion and inversion of whole… CONTINUE READING

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