CRISPR/Cas9‐mediated genome modification in the mollusc, Crepidula fornicata

  title={CRISPR/Cas9‐mediated genome modification in the mollusc, Crepidula fornicata},
  author={K. Perry and J. Henry},
  • K. Perry, J. Henry
  • Published 2015
  • Biology, Medicine
  • genesis
  • The discovery and application of the CRISPR/Cas9 genome editing method has greatly enhanced the ease with which transgenic manipulation can occur. We applied this technology to the mollusc, Crepidula fornicata, and have successfully created transgenic embryos expressing mCherry fused to endogenous β‐catenin. Specific integration of the fluorescent reporter was achieved by homologous recombination with a β‐catenin‐specific donor DNA containing the mCherry coding sequence. This fluorescent gene… CONTINUE READING
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