CRISPR/Cas9‐mediated genome modification in the mollusc, Crepidula fornicata

@article{Perry2015CRISPRCas9mediatedGM,
  title={CRISPR/Cas9‐mediated genome modification in the mollusc, Crepidula fornicata},
  author={K. Perry and J. Henry},
  journal={genesis},
  year={2015},
  volume={53}
}
  • K. Perry, J. Henry
  • Published 2015
  • Biology, Medicine
  • genesis
    • The discovery and application of the CRISPR/Cas9 genome editing method has greatly enhanced the ease with which transgenic manipulation can occur. We applied this technology to the mollusc, Crepidula fornicata, and have successfully created transgenic embryos expressing mCherry fused to endogenous β‐catenin. Specific integration of the fluorescent reporter was achieved by homologous recombination with a β‐catenin‐specific donor DNA containing the mCherry coding sequence. This fluorescent gene… CONTINUE READING
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    References

    SHOWING 1-10 OF 45 REFERENCES
    Biallelic genome modification in F0 Xenopus tropicalis embryos using the CRISPR/Cas system
    • 153
    • Highly Influential
    Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination
    • 129
    • PDF
    Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems
    • 56
    • PDF
    Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering
    • 194
    • PDF
    Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system
    • 940
    • PDF
    Simple and efficient CRISPR/Cas9‐mediated targeted mutagenesis in Xenopus tropicalis
    • 224
    • PDF
    Engineering the Caenorhabditis elegans Genome Using Cas9-Triggered Homologous Recombination
    • 667
    • PDF
    Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis
    • 138
    • PDF
    Heritable gene targeting in the mouse and rat using a CRISPR-Cas system.
    • 523
    TALEN and CRISPR/Cas9-mediated genome editing in the early-branching metazoan Nematostella vectensis.
    • 91
    • PDF