CRISPR, the disruptor

  title={CRISPR, the disruptor},
  author={Heidi Ledford},
A powerful gene-editing technology is the biggest game changer to hit biology since PCR. But with its huge potential come pressing concerns. 
Safety and Security Risks of CRISPR/Cas9
This case study looks into recent developments with regard to the CRISPR/Cas9 and other novel genome editing technologies that are becoming widely available thanks to their low costs and modest
CRISPR/Cas9 and Germline Modification: New Difficulties in Obtaining Informed Consent
This research presents a new approach to selective breeding called “superficial selection”, which aims to find the most efficient way to breed animals with high levels of genetic diversity.
Insertional and Transposon Mutagenesis
This article is a revision of the previous edition article by R.M. Twyman, A. Kohli, volume 1, pp. 369–377, © 2003, Elsevier Ltd.
The emerging role of CRISPR-Cas9 in molecular oncology
Together, they are known as CRISPR-Cas9 or simply “CRISPR” for brevity, the binding of Cas9 causes the CRISpr sequences to become available for editing.
Diversity of CRISPR-Cas immune systems and molecular machines
A plethora of CRISPR molecular machines occur broadly in prokaryotic genomes, with a diversity of Cas nucleases that can be repurposed for various applications.
“I bet you won't”: The science–society wager on gene editing techniques
By proposing a moratorium on human germ line editing using CRISPR, scientists have entered an all‐or‐nothing wager with public opinion. A better approach would be to convene a rational and broad
A Bibliometric Analysis of Research on CRISPR in Social Sciences and Humanities
Abstract Background: The rise of CRISPR not only opens up multiple opportunities for genetic editing but also results in potentially threatening or controversial applications. Objectives: This stud...
The Mechanism of Precise Genome Engineering in Human Cells
This dissertation aims to demonstrate the efforts towards in-situ applicability of EMMARM, which aims to provide real-time information about the response of the immune system to EMTs.
CRISPR/Cas9-Based Genome Editing in Plants.
The technical features of the plant CRISPR/Cas9-based genome editing system and its applications in plant functional genomics studies and genetic improvement are introduced.
Is CRISPR a Security Threat?
Since 2012, the new gene editing technique called CRISPR took the world by storm because theoretically it can be used to edit any organism quickly, precisely, and at low cost. Because of these


CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes
It is found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences, and CRISPR provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.
A Candida albicans CRISPR system permits genetic engineering of essential genes and gene families
This work describes a C. albicans CRISPR system that overcomes many of the obstacles to genetic engineering in this organism and provides a new window into the biology of this pathogen.
Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype
This study indicates that CRISPR-Cas9–mediated genome editing is possible in adult animals and has potential for correction of human genetic diseases.
RNA-Guided Human Genome Engineering via Cas9
The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
RNA-programmed genome editing in human cells
It is shown here that Cas9 assembles with hybrid guide RNAs in human cells and can induce the formation of double-strand DNA breaks at a site complementary to the guide RNA sequence in genomic DNA.
Multiplex Genome Engineering Using CRISPR/Cas Systems
Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes
It is found that CRISPR/Cas9 could effectively cleave the endogenous β-globin gene (HBB), however, the efficiency of homologous recombination directed repair (HDR) of HBB was low and the edited embryos were mosaic.
In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system
An efficient method to induce specific chromosomal rearrangements in vivo using viral-mediated delivery of the CRISPR/Cas9 system to somatic cells of adult animals is described, substantially expands the ability to model human cancers in mice and potentially in other organisms.
Regulating gene drives
Potential beneficial uses of gene drives include reprogramming mosquito genomes to eliminate malaria, reversing the development of pesticide and herbicide resistance, and locally eradicating invasive species, however, drives may present environmental and security challenges as well as benefits.