COPI recruitment is modulated by a Rab1b-dependent mechanism.

  title={COPI recruitment is modulated by a Rab1b-dependent mechanism.},
  author={Cecilia Alvarez and Rafael Garcia-Mata and Elizabeth S. Brandon and Elizabeth S. Sztul},
  journal={Molecular biology of the cell},
  volume={14 5},
The small GTPase Rab1b is essential for endoplasmic reticulum (ER) to Golgi transport, but its exact function remains unclear. We have examined the effects of wild-type and three mutant forms of Rab1b in vivo. We show that the inactive form of Rab1b (the N121I mutant with impaired guanine nucleotide binding) blocks forward transport of cargo and induces Golgi disruption. The phenotype is analogous to that induced by brefeldin A (BFA): it causes resident Golgi proteins to relocate to the ER and… CONTINUE READING