CONTINUOUS ANAEROBIOSIS FOR CULTIVATION OF SPIROCHETES.

@article{Rosebury1964CONTINUOUSAF,
  title={CONTINUOUS ANAEROBIOSIS FOR CULTIVATION OF SPIROCHETES.},
  author={Theodor Rosebury and Joseph B. Reynolds},
  journal={Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine},
  year={1964},
  volume={117},
  pages={
          813-5
        }
}
SummaryApparatus and procedures have been developed for bacteriological work based on a vacuum-tight glove box with lock, in which an anaerobic working atmosphere has been maintained by evacuation, followed by removal of residual and diffusing oxygen with hydrogen and a catalyst that does not require pre-heating. By these means anaerobic spiro-chetes have appeared regularly as surface colonies on agar streaked from primary cultures, and have been isolated routinely. 
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