• Biology
  • Published 2001

CONSTRUCTION OF THE PLASMID pKChiA AND pMchiA CARRYING CHITINASE GENE AND THEIR EXPRESSION IN E.coli

@inproceedings{Zhi2001CONSTRUCTIONOT,
  title={CONSTRUCTION OF THE PLASMID pKChiA AND pMchiA CARRYING CHITINASE GENE AND THEIR EXPRESSION IN E.coli},
  author={Ye Zhi},
  year={2001}
}
A 1.8 kb HinfI fragment carrying the chitinase gene(chiA) from Serratia marcescens was isolated from the plasmid pLCHIA and was inserted into the SmaI site downstream of the strong Ptac promoter in the expression vector pKK223-3,yielding the plasmid pKChiA.The 2.1 kb PtacChiA fusion fragment was excised by BamHI from the plasmid pKChiA and was inserted into the single BamHI site present within the plasmid pMC71A,thus generating the plasmid pMChiA.High levels of chitinase were produced by the E… CONTINUE READING