COMBO-FISH: specific labeling of nondenatured chromatin targets by computer-selected DNA oligonucleotide probe combinations.

@article{Hausmann2003COMBOFISHSL,
  title={COMBO-FISH: specific labeling of nondenatured chromatin targets by computer-selected DNA oligonucleotide probe combinations.},
  author={Michael Hausmann and Ralph Winkler and Georg Hildenbrand and Jutta Finsterle and Andrea Weisel and Alexander Rapp and Eberhard Schmitt and Siegfried Janz and Christoph Cremer},
  journal={BioTechniques},
  year={2003},
  volume={35 3},
  pages={
          564-70, 572-7
        }
}
Here we present the principle of fluorescence in situ hybridization (FISH) with combinatorial oligonucleotide (COMBO) probes as a new approach for the specific labeling of genomic sites. COMBO-FISH takes advantage of homopurine/homopyrimidine oligonucleotides that form triple helices with intact duplex genomic DNA, without the need for prior denaturation of the target sequence that is usually applied for probe binding in standard FISH protocols. An analysis of human genome databases has shown… CONTINUE READING

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