COG lobe B sub-complex engages v-SNARE GS15 and functions via regulated interaction with lobe A sub-complex

Abstract

The conserved oligomeric Golgi (COG) complex is a peripheral membrane protein complex which orchestrates tethering of intra-Golgi vesicles. We found that COG1-4 (lobe A) and 5-8 (lobe B) protein assemblies are present as independent sub-complexes on cell membranes. Super-resolution microscopy demonstrates that COG sub-complexes are spatially separated on the Golgi with lobe A preferential localization on Golgi stacks and the presence of lobe B on vesicle-like structures, where it physically interacts with v-SNARE GS15. The localization and specific interaction of the COG sub-complexes with the components of vesicle tethering/fusion machinery suggests their different roles in the vesicle tethering cycle. We propose and test a novel model that employs association/disassociation of COG sub-complexes as a mechanism that directs vesicle tethering at Golgi membranes. We demonstrate that defective COG assembly or restriction of tethering complex disassembly by a covalent COG1-COG8 linkage is inhibitory to COG complex activity, supporting the model.

DOI: 10.1038/srep29139

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Cite this paper

@inproceedings{Willett2016COGLB, title={COG lobe B sub-complex engages v-SNARE GS15 and functions via regulated interaction with lobe A sub-complex}, author={Rose A Willett and Jessica Bailey Blackburn and Leslie K. Climer and Irina Pokrovskaya and Tetyana A Kudlyk and Wei Wang and Vladimir Lupashin}, booktitle={Scientific reports}, year={2016} }