COBRA: a sensitive and quantitative DNA methylation assay.

  title={COBRA: a sensitive and quantitative DNA methylation assay.},
  author={Zhongxian Xiong and Peter W. Laird},
  journal={Nucleic acids research},
  volume={25 12},
We report here on a quantitative technique called COBRA to determine DNA methylation levels at specific gene loci in small amounts of genomic DNA. Restriction enzyme digestion is used to reveal methylation-dependent sequence differences in PCR products of sodium bisulfite-treated DNA as described previously. We show that methylation levels in the original DNA sample are represented by the relative amounts of digested and undigested PCR product in a linearly quantitative fashion across a wide… CONTINUE READING
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