CMP kinase from Escherichia coli is structurally related to other nucleoside monophosphate kinases.

@article{Bucurenci1996CMPKF,
  title={CMP kinase from Escherichia coli is structurally related to other nucleoside monophosphate kinases.},
  author={Nadia Bucurenci and Hiroshi Sakamoto and Pierre Briozzo and Nicolae Palibroda and L Serina and R S Sarfati and Gilles Labesse and Gilbert Briand and Antoine Danchin and Octavian B{\^a}rzu and Anne Marie Gilles},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 5},
  pages={2856-62}
}
CMP kinase from Escherichia coli is a monomeric protein of 225 amino acid residues. The protein exhibits little overall sequence similarities with other known NMP kinases. However, residues involved in binding of substrates and/or in catalysis were found conserved, and sequence comparison suggested conservation of the global fold found in adenylate kinases or in several CMP/UMP kinases. The enzyme was purified to homogeneity, crystallized, and analyzed for its structural and catalytic… CONTINUE READING