CLAG 9 is located in the rhoptries of Plasmodium falciparum


Clag 9, a gene located on chromosome 9 of Plasmodium falciparum has previously been associated with the cytoadherence of parasitized erythrocytes to CD36. This gene is part of a multi-gene family found in all Plasmodium species studied to date. Using data from the Malaria Genome Sequencing Project, peptides specific for clag 9 were designed, synthesized and used to immunize mice. This antisera was used in Western blotting and immunofluorescence experiments to determine the cellular localization of CLAG 9 in the parasitized erythrocyte. Co-localization using immunofluorescence of wildtype and knockout parasites unequivocally shows that CLAG 9 is localized to the rhoptry organelles of P. falciparum.

DOI: 10.1007/s00436-004-1098-4

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@article{Gardiner2004CLAG9I, title={CLAG 9 is located in the rhoptries of Plasmodium falciparum}, author={Donald L. Gardiner and Tobias Spielmann and Matthew W A Dixon and Paula L. Hawthorne and Mar{\'i}a Rodr{\'i}guez Ortega and Karen L. Anderson and Tina S. Skinner-Adams and David J. Kemp and Katharine R. Trenholme}, journal={Parasitology Research}, year={2004}, volume={93}, pages={64-67} }