CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.

@article{Iverson2016CIDARMI,
  title={CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.},
  author={Sonya V. Iverson and Traci Haddock and Jacob Beal and Douglas M Densmore},
  journal={ACS synthetic biology},
  year={2016},
  volume={5 1},
  pages={
          99-103
        }
}
Multipart and modular DNA part libraries and assembly standards have become common tools in synthetic biology since the publication of the Gibson and Golden Gate assembly methods, yet no multipart modular library exists for use in bacterial systems. Building upon the existing MoClo assembly framework, we have developed a publicly available collection of modular DNA parts and enhanced MoClo protocols to enable rapid one-pot, multipart assembly, combinatorial design, and expression tuning in… CONTINUE READING

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