CHO cells provide access to novel N-glycans and developmentally regulated glycosyltransferases.

  title={CHO cells provide access to novel N-glycans and developmentally regulated glycosyltransferases.},
  author={Pamela Stanley and T Shantha Raju and Mantu Bhaumik},
  volume={6 7},
Chinese hamster ovary (CHO) cells express only a subset of the glycosyltransferases activities known to exist. They do not express several fucosyltransferases, galactosyltransferases, sialyltransferases or N-acetylglucosaminyltransferases. However, following mutagenesis or transfection with large amounts of DNA, rare mutants that express a transferase activity de novo have been obtained. The first CHO mutant of this type was LEC10, which expresses the N-acetylglucosaminyltransferase, GlcNAc… CONTINUE READING


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