Corpus ID: 165266602

CHARACTERIZATION OF A RECOMBINANT HUMAN FUSION PROTEIN IL6/ IL2 (CH925)

@inproceedings{Chunhua1994CHARACTERIZATIONOA,
  title={CHARACTERIZATION OF A RECOMBINANT HUMAN FUSION PROTEIN IL6/ IL2 (CH925)},
  author={Chun-hua and Zhao and Pei-Hsien and Tang and Jia-xi and Wang and Ning and Mao and Feizi and Jiang and Xiu-Sen and Li and Xiu-zhen and L{\'i}u and Ming-wei and Zhang and Yun-Fang and Ren and De-lin and Beijing},
  year={1994}
}
An expression vector encoding the fusion protein IL6 /IL2 has been constructed.After the flexible linker was synthesized and conjugated with IL2 gene fragment by PCRamplification, the IL6 gene fragment was unidirectionally inserted into the upstream tolinker-IL2 sequence. The molecule of IL6-linker-IL2 fusion gene named E.coli DH5 α/pfIL6/2 was cloned and identified by DNA sequencing. The expressed protein namedCH925 showed a strong band amounting to 32% of total cell protein on SDS-PAGE,with a… Expand
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