CHAPTER 2 C Ruminant DNA detection : Real-time PCR Detection of Ruminant DNA

@inproceedings{MendozaRomero2003CHAPTER2C,
  title={CHAPTER 2 C Ruminant DNA detection : Real-time PCR Detection of Ruminant DNA},
  author={Luis Mendoza-Romero and Edward L C Verkaar and P. J. M. Savelkoul and Arnold Catsburg and Henk J. M. Aarts and Jaap B. Buntjer and Johannes A Lenstra},
  year={2003}
}
In order to control the spread of transmissible spongiform encephalopathy (TSE), several DNA methods have been described for the detection of the species origin of meat-andbone-meal. Most of these methods are based on the amplification of a mitochondrial DNA segment. We have developed a semi-quantitative method based on real-time PCR for detection of ruminant DNA, targeting an 88-bp segment of the ruminant short interspersed nuclear element (SINE) Bov-A2. This method is specific for ruminants… CONTINUE READING

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