CENP-E is a putative kinetochore motor that accumulates just before mitosis

@article{Yen1992CENPEIA,
  title={CENP-E is a putative kinetochore motor that accumulates just before mitosis},
  author={Tim J. Yen and Gang Li and Bruce T. Schaar and I Szilak and Don W. Cleveland},
  journal={Nature},
  year={1992},
  volume={359},
  pages={536-539}
}
THE mechanics of chromosome movement, mitotic spindle assembly and spindle elongation have long been central questions of cell biology1. After attachment in prometaphase of a micro-tubule from one pole, duplicated chromosome pairs travel towards the pole in a rapid but discontinuous motion2,3. This is followed by a slower congression towards the midplate as the chromosome pair orients with each kinetochore attached to the microtubules from the nearest pole. The pairs disjoin at anaphase and… 

Leaving no-one behind: how CENP-E facilitates chromosome alignment

The function of CENp-E in chromosome congression, the pathways that contribute to CENP-E loading at the kinetochore, and how CENE-E activity is regulated during mitosis are reviewed.

A new role for motor proteins as couplers to depolymerizing microtubules

Kinetochores may use motor proteins as the molecular interface that couples microtubule depolymerization to chromosome movement, implying that the force for poleward chromosome movement is predominantly generated at kinetochores.

Mechanism of the mitotic kinesin CENP-E in tethering kinetochores to spindle microtubules

It is proposed that the highly processive microtubule-dependent motor activity of CENP-E serves not only to power chromosome congression, but also to provide a flexible, motile tether linking kinetochores to dynamic spindle microtubules.

Anaphase A: Melting Microtubules Move Chromosomes toward Spindle Poles

Molecular mechanistic models for how load-bearing attachments are maintained to disassembling microtubule ends, and how the forces are generated to drive pac-man and flux-based movements, are discussed.

Microtubule detyrosination guides chromosomes during mitosis

It is found that congression of pole-proximal chromosomes depended on specific posttranslational detyrosination of spindle microtubules that point to the equator, and CENP-E–driven chromosome congression is guided by microtubule detyromination.

CENP-E Function at Kinetochores Is Essential for Chromosome Alignment

The combined data show that CENP-E provides kinetochore functions that are essential for monopolar chromosomes to establish bipolar connections and for chromosomes with connections to both spindle poles to align at the spindle equator.

Anaphase A: Disassembling Microtubules Move Chromosomes toward Spindle Poles

This chapter begins by summarizing classical observations of chromosome movements, which support the current understanding of anaphase mechanisms, and molecular mechanistic models for how load-bearing attachments are maintained to disassembling microtubule ends, and how the forces are generated to drive these disassembly-coupled movements are discussed.

Cytoplasmic dynein is required for poleward chromosome movement during mitosis in Drosophila embryos

Time-lapse confocal microscopy is used to visualize, in real time, kinetochore and chromatid movements in living Drosophila embryos in the presence and absence of specific inhibitors of cytoplasmic dynein, showing that dyneins are essential for poleward chromosome motility throughout mitosis in Drosophile embryos.
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