CENP-E as an Essential Component of the Mitotic Checkpoint In Vitro

@article{Abrieu2000CENPEAA,
  title={CENP-E as an Essential Component of the Mitotic Checkpoint In Vitro},
  author={Ariane Abrieu and Jason Kahana and K. Wood and Don W Cleveland},
  journal={Cell},
  year={2000},
  volume={102},
  pages={817-826}
}
Accurate chromatid separation is monitored by a checkpoint mechanism that delays anaphase onset until all centromeres are correctly attached to the mitotic spindle. Using Xenopus egg extracts, the kinetochore-associated microtubule motor protein CENP-E is now found to be required for establishing and maintaining this checkpoint. When CENP-E function is disrupted by immunodepletion or antibody addition, extracts fail to arrest in response to spindle damage. Mitotic arrest can be restored by… CONTINUE READING