CENP-E Function at Kinetochores Is Essential for Chromosome Alignment

@article{Schaar1997CENPEFA,
  title={CENP-E Function at Kinetochores Is Essential for Chromosome Alignment },
  author={B. T. Schaar and G.K.T. Chan and Paul F Maddox and E. D. Salmon and T J Yen},
  journal={The Journal of Cell Biology},
  year={1997},
  volume={139},
  pages={1373 - 1382}
}
CENP-E is a kinesin-like protein that binds to kinetochores and may provide functions that are critical for normal chromosome motility during mitosis. To directly test the in vivo function of CENP-E, we microinjected affinity-purified antibodies to block the assembly of CENP-E onto kinetochores and then examined the behavior of these chromosomes. Chromosomes lacking CENP-E at their kinetochores consistently exhibited two types of defects that blocked their alignment at the spindle equator… CONTINUE READING
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