CEDIA, a new homogeneous immunoassay system.

@article{Henderson1986CEDIAAN,
  title={CEDIA, a new homogeneous immunoassay system.},
  author={Daniel R. Henderson and Stanford B Friedman and Jeffrey D Harris and W. B. Manning and M A Zoccoli},
  journal={Clinical chemistry},
  year={1986},
  volume={32 9},
  pages={1637-41}
}
Genetic engineering of beta-galactosidase (EC 3.2.1.23) has led to the development of a new homogeneous assay system, CEDIA. The Z gene of the lac operon of Escherichia coli encodes a large enzymatically inactive polypeptide that spontaneously aggregates and folds to form active beta-galactosidase. Using recombinant DNA techniques, we have been able to engineer beta-galactosidase protein into a large polypeptide (an enzyme acceptor, EA) and a small polypeptide (an enzyme donor, ED). The EAs and… CONTINUE READING
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