CD95 ligand (CD95L) immunohistochemistry: a critical study on 12 antibodies

  title={CD95 ligand (CD95L) immunohistochemistry: a critical study on 12 antibodies},
  author={J{\"o}rn Str{\"a}ter and Henning Walczak and Cornelia Hasel and Ingo Melzner and Frank Leithaeuser and Peter L Moeller},
  journal={Cell Death and Differentiation},
In recent years, some studies on the expression of CD95(Fas/APO-1) ligand (CD95L) in tissues or cells raised concerns about the specificity of the antibodies used. We therefore tested 12 CD95L antibodies for their reliability in immunocyto/histochemistry by (i) staining CD95L-transfected and control CV-1/EBNA cells and (ii) comparing staining patterns in immunohistochemically labeled tissue sections with the localization of CD95L+ cells in in situ hybridization. While G247-4, NOK-1, NOK-2, 4H9… CONTINUE READING


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