CD9 of mouse brain is implicated in neurite outgrowth and cell migration in vitro and is associated with the α6/β1 integrin and the neural adhesion molecule L1

@article{Schmidt1996CD9OM,
  title={CD9 of mouse brain is implicated in neurite outgrowth and cell migration in vitro and is associated with the $\alpha$6/$\beta$1 integrin and the neural adhesion molecule L1},
  author={C. Schmidt and V. K{\"u}nemund and E. Wintergerst and B. Schmitz and M. Schachner},
  journal={Journal of Neuroscience Research},
  year={1996},
  volume={43}
}
We describe here a novel monoclonal antibody (mab INTRODUCTION H6) which recognizes CD9, an integral cell surface constituent previously described in cells of the hematopoietic lineage and involved in the aggregation of platelets. Mab H6 was raised against membranes of immature mouse astrocytes and reacted with a protein of 25–27 kD in detergent extracts of adult mouse brain membranes. Sequence analysis of the N‐terminal amino acids revealed an identity of 96% with CD9 from mouse kidney. CD9… Expand
An Antibody to the Tetraspan Membrane Protein CD9 Promotes Neurite Formation in a Partially α3β1 Integrin-Dependent Manner
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It is shown that B2C11, an anti-CD9 antibody that has been shown previously to activate Schwann cells in vitro, promotes robust neurite outgrowth from sympathetic neurons that is greater than that on other antibody surfaces and is comparable to neuriteOutgrowth on a collagen substratum. Expand
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References

SHOWING 1-10 OF 94 REFERENCES
Monoclonal M6 antibody interferes with neurite extension of cultured neurons.
TLDR
The unusually short and flattened appearance of neurites in these cultures suggests that the M6 antibody selectively affects neurite extension, and indicates that the overall extension of the neurite appears to be inhibited. Expand
Neurite outgrowth patterns in cerebellar microexplant cultures are affected by antibodies to the cell surface glycoprotein L1
TLDR
The findings suggest that L1 antigen not only plays a role in adhesion of isolated neural cell bodies and migration of granule cell neurons in the early postnatal mouse cerebellar cortex, but also in neurite outgrowth and fasciculation. Expand
Distribution of CD9 in the developing and mature rat nervous system
  • S. Tole, P. Patterson
  • Biology, Medicine
  • Developmental dynamics : an official publication of the American Association of Anatomists
  • 1993
CD9 is a cell surface protein implicated in intercellular signaling that has been identified in selected cell types of the hematopoietic system. To begin a study of the role of CD9 in the developingExpand
Detection of a cell surface antigen found on rat peripheral nervous system neurons and multiple glia: Astrocytes, oligodendrocytes, and Schwann cells
TLDR
The B2C11 antigen is enriched in neural tissues, where it is found on the surfaces of a unique set of neuronal and glial cells. Expand
Subclass of astroglia in mouse cerebellum recognized by monoclonal antibody.
TLDR
The developmental regulation of M 1 expression and the abnormal expression of M1 in weaver mutant cerebella suggest that M1 may be a useful marker for astroglial maturation and differentiation. Expand
A new brain cell surface glycoprotein identified by monoclonal antibody
TLDR
The availability of a monoclonal antibody produced by a stable hybridoma line will be a powerful tool in attempts to purify the BSP-3 antigen and to elucidate its function. Expand
Comparison of two cell surface molecules involved in neural cell adhesion.
TLDR
The results indicate that two chemically and histochemically distinct cell surface polypeptides can contribute to the calcium‐independent adhesiveness of neural cells, and suggest that their differential expression might cause adhesive specificity among cells of developing neural tissues. Expand
Differential inhibition of neurone–neurone, neurone–astrocyte and astrocyte–astrocyte adhesion by L1, L2 and N-CAM antibodies
TLDR
It is reported that LI antigen promotes neurone–neurone adhesion and the L2 carbohydrate epitope shared between the two adhesion molecules seems to be involved in neurones and glia, and acts in a more than additive manner in N-CAM-mediated neurone-neur one adhesion. Expand
Immunoelectron microscopic localization of neural cell adhesion molecules (L1, N-CAM, and MAG) and their shared carbohydrate epitope and myelin basic protein in developing sciatic nerve
TLDR
These studies show that axon-Schwann cell interactions are characterized by the sequential appearance of cell adhesion molecules and MBP apparently coordinated in time and space. Expand
Immunocytological and biochemical characterization of a new neuronal cell surface component (L1 antigen) which is involved in cell adhesion.
TLDR
During cerebellar development L1 antigen is detectable on tetanus toxin‐positive cells as early as embryonic day 13 after 3 days in culture and in the adult cerebellum, where it is predominantly localized in the molecular layer and around Purkinje cells. Expand
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