CD8+ T cell concentration determines their efficiency in killing cognate antigen–expressing syngeneic mammalian cells in vitro and in mouse tissues

@inproceedings{Budhu2010CD8TC,
  title={CD8+ T cell concentration determines their efficiency in killing cognate antigen–expressing syngeneic mammalian cells in vitro and in mouse tissues},
  author={Sadna Budhu and John D. Loike and Ashley Pandolfi and Soo Han and Geoffrey Catalano and A. C. Constantinescu and Raphael Clynes and Samuel C. Silverstein},
  booktitle={The Journal of experimental medicine},
  year={2010}
}
We describe a quantitative model for assessing the cytolytic activity of antigen-specific CD8+ T cells in vitro and in vivo in which the concentration of antigen-specific CD8+ T cells determines the efficiency with which these cells kill cognate antigen-expressing melanoma cells in packed cell pellets, in three-dimensional collagen-fibrin gels in vitro, and in established melanomas in vivo. In combination with a clonogenic assay for melanoma cells, collagen-fibrin gels are 4,500-5,500-fold more… CONTINUE READING

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