CD43, a molecule defective in Wiskott-Aldrich syndrome, binds ICAM-1

  title={CD43, a molecule defective in Wiskott-Aldrich syndrome, binds ICAM-1},
  author={Yvonne Rosenstein and John K. Park and William C. Hahn and Fred S. Rosen and Barbara E. Bierer and Steven J. Burakoff},
THE protein CD43 (also known as sialophorin, leukosialin, large sialoglycoprotein or gp115) is expressed on the surface of T lymphocytes, monocytes, neutrophils, platelets and some B lymphocytes1–6. Expression of CD43 is deficient and/or defective in the X-chromosome-linked immunodeficiency disorder Wiscott-Aldrich syndrome7, suggesting that CD43 might have a role in T-cell activation. We have shown that expression of human CD43 in an HLA-DR-specific murine T-cell hybridoma enhances the antigen… 
Negative regulation of T-cell adhesion and activation by CD43
It is concluded that CD43 negatively regulates T-cell activation and adhesion and is important for viral clearance.
P-Selectin Glycoprotein Ligand-1 Negatively Regulates T-Cell Immune Responses1
It is demonstrated that the intracellular domain of PSGL-1 or CD43 is required for suppressing proliferation but not adhesion, and this results reveal a novel regulatory role for PS GL-1 in T cell adhesion and proliferation and suggest that PSGL -1 negatively regulates T cell immune responses in vivo.
Molecular Mechanisms Involved in CD43-mediated Apoptosis of TF-1 Cells
Engagement of CD43 may, presumably through the repressing transcription, initiate a Bad-dependent apoptotic pathway, which is suggested to be responsible for apoptosis of hematopoietic progenitors.
CD43 (leukosialin, sialophorin) expression is differentially regulated by retinoic acids
Evidence is provided that the vitamin A metabolites all‐trans and 13‐cis retinoic acid up‐regulate CD43 on human leukemic (HMC‐1) mast cells, as determined by flow cytometry, Western blot analysis, and by semiquantitative reverse transcriptase‐polymerase chain reaction.
Aberrant expression of the major sialoglycoprotein (CD43) on the monocytes of patients with myelodysplastic syndromes
Abstract CD43, a sialylated glycoprotein expressed on the surface of most hematopoietic cells, has been implicated in cell adhesion and signaling. The reduced expression of this antigen in patients
Disregulation of leukosialin (CD43, Ly48, sialophorin) expression in the B-cell lineage of transgenic mice increases splenic B-cell number and survival.
The alteration of the temporal expression, or "disregulation," of a gene in transgenic mice provides a general strategy for elucidating the in vivo role of other molecules involved in cell signaling and adhesion.
CD43 is a ligand for E-selectin on CLA+ human T cells.
The identification and characterization of CD43 as a T-cell E-selectin ligand distinct from PSGL-1 expands the role ofCD43 in the regulation of T- cell trafficking and provides new targets for the modulation of immune functions in skin.
Downregulation of CD43 in RAEB and RAEB‐T patients. Report of 3 cases
Three cases of patients with myelodysplastic syndromes are reported in which acquired severe deficiency of the CD43 antigen on the surface of most hemopoietic cells was observed and the mechanism of downregulation of the gene was revealed.
LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147.
CD43 Functions as a Ligand for E-Selectin on Activated T Cells1
Results suggest that CD43, when modified by a specific set of glycosyltranferases, can function as an E-selectin ligand and therefore potentially mediate activated T cell migration into inflamed sites.


Enhancement of T-cell activation by the CD43 molecule whose expression is defective in Wiskott–Aldrich syndrome
It is observed that CD43 enhances the antigen-specific activation of T cells and that the intracellular domain of CD43, which is hyperphosphorylated during T-cell activation19–21, is required for this function.
Mechanism of mononuclear cell activation by an anti-CD43 (sialophorin) agonistic antibody.
It is concluded that CD43 is functionally coupled to the phospholipase C/phosphoinositides signaling pathway in human T cells, and a mutant derived from the leukemic T cell line HPB-ALL that was severely defective in TCR/CD3 surface expression and signaling nevertheless had normal CD43 surface expression compared with the parent cell line.
Sialophorin, a surface sialoglycoprotein defective in the Wiskott- Aldrich syndrome, is involved in human T lymphocyte proliferation
Observations suggest that sialophorin functions in T cell activation in patients with the X-linked immunodeficiency Wiskott-Aldrich syndrome.
The lymphocyte function-associated LFA-1, CD2, and LFA-3 molecules: cell adhesion receptors of the immune system.
This review focuses on LFAI, CD2, and LFA-3, which appear to enhance antigen-specific functions by acting as cell adhesion molecules and the role of CD4 and CD8 is reviewed by Littman.
Interaction of CD2 with its ligand lymphocyte function-associated antigen-3 induces adenosine 3',5'-cyclic monophosphate production in T lymphocytes.
The ability of purified LFA-3 and anti-CD2 mAb to induce changes in intracellular cAMP content in murine Ag-specific T cell hybridomas that stably express wild-type and mutated human CD2 molecules is studied and suggests that CD2/LFA- 3 interactions may regulate T cell function at least in part through the generation of intrace cellular cAMP.
On the species specificity of the interaction of LFA-1 with intercellular adhesion molecules.
Evidence for multiple counter-receptors for LFA-1 in the mouse as well as in the human is provided and increased adhesiveness for ICAM-1 stimulated by phorbol esters could be demonstrated for hybrid L FA-1 molecules with human alpha and murine beta subunits.
ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18)
It is concluded that ICAM-1 is a counter receptor for Mac-1 and that this receptor pair is responsible, in part, for the adhesion between stimulated neutrophils and stimulated endothelial cells.
Expression of the T-cell surface molecule CD2 and an epitope-loss CD2 mutant to define the role of lymphocyte function-associated antigen 3 (LFA-3) in T-cell activation.
To define the role of the CD2-lymphocyte function-associated antigen 3 (LFA-3) interaction in T-cell activation, we have expressed a cDNA encoding the human CD2 molecule in a murine antigen-specific
A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1.
It is proposed that ICAM-1 may be a ligand in many, but not all, LFA-1-dependent adhesion reactions.
Induction of aggregation and enhancement of proliferation and IL-2 secretion in human T cells by antibodies to CD43.
The results indicate that regulatory signals, which may function to modify homo- or heterotypic T cell adhesion as well as autocrine production of IL-2, can be transduced through CD43.