CD4/major histocompatibility complex class II interaction analyzed with CD4‐ and lymphocyte activation gene‐3 (LAG‐3)‐Ig fusion proteins

@article{Huard1995CD4majorHC,
  title={CD4/major histocompatibility complex class II interaction analyzed with CD4‐ and lymphocyte activation gene‐3 (LAG‐3)‐Ig fusion proteins},
  author={Bertrand Huard and Philippe Prigent and Muriel Tournier and Denis Bruniquel and Frederic Triebel},
  journal={European Journal of Immunology},
  year={1995},
  volume={25}
}
We analyzed CD4 major histocompatibility complex (MHC) class II interactions with CD4 and lymphocyte activation gene (LAG)‐3 recombinant fusion proteins termed CD4Ig and LAG‐3Ig. CD4Ig bound MHC class II molecules expressed on the cell surface only when used in the micromolar range. This weak CD4Ig binding was specific, since it was inhibited by anti‐CD4 and anti‐MHC class II mAb. LAG‐3Ig bound MHC class II molecules with intermediate avidity (Kd = 60 nM at 37°C). Using LAG‐3Ig as a competitor… Expand
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