CCR3 as a single selection marker compared to CD123/HLADR to isolate basophils in flow cytometry: Some comments

@article{Chirumbolo2011CCR3AA,
  title={CCR3 as a single selection marker compared to CD123/HLADR to isolate basophils in flow cytometry: Some comments},
  author={Salvatore Chirumbolo and Riccardo Ortolani and Antonio Vella},
  journal={Cytometry Part A},
  year={2011},
  volume={79A}
}
INTRODUCTION Flow cytometry of activated basophils is extensively used to assess cell response to allergens or to soluble agonists and has immediately proved a successful approach for studying the function of basophils in vitro (1). The mechanism of expression of various membrane proteins is the basis of basophil activation tests (BATs). Several questions and influencing factors on the analytical feasibility and reliability of BATs still remain and generally the potentials and pitfall of these… 
Commentary: The Expression of CD123 Can Decrease with Basophil Activation: Implications for the Gating Strategy of the Basophil Activation Test
TLDR
It is shown that, during cellular activation, the expression of membrane CD123, the alpha subunit of the interleukin-3 (IL-3) receptor, is downregulated, leading to a possible loss in the electronic capture of basophils and hence a false negative outcome of the activation test.
The expression of CD123 can decrease with basophil activation: implications for the gating strategy of the basophil activation test
TLDR
The use of CD203c before gating on CD123+/HLA-DR− cells or in isolation ensures the identification of the entire basophils population and accurate assessment of basophil activation, with important diagnostic implications.
CD164 as a Basophil Activation Marker.
TLDR
Although CD164 upregulation as a marker of basophil activation is a promising and powerful diagnostic tool, it still requires a lot of both basic research and comparative studies with older and well known markers, in order to select the best of them.
Using a CD45dim/CD123bright/HLA-DRneg phenotyping protocol to gate basophils in FC for airway allergy. CD123 does not decrease.
TLDR
The methodological aspects of the use of the protocol adopting the panel CD45dim/CD123bright/HLA-DRneg to gate basophils in flow cytometry are described, trying to highlight the main biases related to an incorrect use of this protocol.
Basophil Activation Test for Chronic Urticaria
TLDR
The authors identified the need to add the CD63 marker to the basophil activation test (BAT), as CD203c expression is dependent on primed basophils and that CD63 should be reported as a fundamental marker to assess basophIL activation.
Evaluation of CCR3 as a basophil activation marker.
TLDR
Overall the data show that CCR3 demonstrates adequate sensitivity but weak specificity in its ability to reliably identify histamine-releasing activated basophils.
Basophil Markers for Identification and Activation in the Indirect Basophil Activation Test by Flow Cytometry for Diagnosis of Autoimmune Urticaria
TLDR
The indirect basophil activation test using flow cytometry is a promising tool for autoimmune urticaria diagnosis and a combination of CD123 and CCR3 is recommended, while CD123 alone and CD63 alone on primed basophils may be used as an alternative.
Basophil Activation Tests Based on CD193 Marker in Dipyrone Allergy
TLDR
It apppears quite difficult to attribute this sensitivity improvement to superior BAT performance (at least in regards to the phenotyping protocol), although it may be significant when CD63% is close to the threshold.
Basophil Activation Test in Allergy: Time for an Update?
  • S. Chirumbolo
  • Biology
    International Archives of Allergy and Immunology
  • 2012
TLDR
BAT is certainly a useful technique, also for isolated cases of hypersensitivity to various other compounds and drugs, and an update of its application is certainly an interesting topic to expand the debate on allergy diagnosis.
State-of-the-art review about basophil research in immunology and allergy: is the time right to treat these cells with the respect they deserve?
  • S. Chirumbolo
  • Biology, Medicine
    Blood transfusion = Trasfusione del sangue
  • 2012
TLDR
In clinical settings, basophils are a practical and convenient alternative for the diagnosis of allergy because they are much more accessible than tissue-resident mast cells and it is suffi cient to take a blood sample to obtain them.
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