CAL1, a gene required for activity of chitin synthase 3 in Saccharomyces cerevisiae

  title={CAL1, a gene required for activity of chitin synthase 3 in Saccharomyces cerevisiae},
  author={M H Valdivieso and Pieternella C. Mol and Jacqui A Shaw and E. Cabib and Angel Dur{\'a}n},
  journal={The Journal of Cell Biology},
  pages={101 - 109}
The CAL1 gene was cloned by complementation of the defect in Calcofluor-resistant calR1 mutants of Saccharomyces cerevisiae. Transformation of the mutants with a plasmid carrying the appropriate insert restored Calcofluor sensitivity, wild-type chitin levels and normal spore maturation. Southern blots using the DNA fragment as a probe showed hybridization to a single locus. Allelic tests indicated that the cloned gene corresponded to the calR1 locus. The DNA insert contains a single open… CONTINUE READING
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