C2C12 myoblastoma cell differentiation and proliferation is stimulated by androgens and associated with a modulation of myostatin and Pax7 expression.

@article{Diel2008C2C12MC,
  title={C2C12 myoblastoma cell differentiation and proliferation is stimulated by androgens and associated with a modulation of myostatin and Pax7 expression.},
  author={Patrick Diel and D Baadners and K. Schl{\"u}pmann and Martina Velders and J P Schwarz},
  journal={Journal of molecular endocrinology},
  year={2008},
  volume={40 5},
  pages={
          231-41
        }
}
Androgens are modulators of skeletal muscle adaptation and regeneration processes. The control of satellite cell activity is a key mechanism during this process. In this study, we analyzed the ability of dihydrotestosterone (DHT) and anabolic steroids to induce and modulate the differentiation of C2C12 myoblastoma cells toward myotubes. C2C12 cells were dose-dependently treated with DHT and anabolic steroids. The time-dependent effects on differentiation were measured and correlated with the… Expand
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Myostatin stimulates myosatellite cell differentiation in a novel model system: evidence for gene subfunctionalization.
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  • Biology, Medicine
  • American journal of physiology. Regulatory, integrative and comparative physiology
  • 2012
TLDR
The results conflict with studies using mammalian cell lines and suggest, alternatively, that myostatin is a positive, not negative, regulator of myosatellite cell differentiation, and the primary rainbow trout myos satellite cell culture system appears to be more physiologically relevant. Expand
Muscle-specific androgen receptor deletion shows limited actions in myoblasts but not in myofibers in different muscles in vivo.
TLDR
The pattern of expression of genes including c-myc, Fzd4 and Igf2 is associated with androgen-dependent changes in muscle mass; therefore, these genes are likely to be mediators of anabolic actions of androgens. Expand
Ornithine decarboxylase is upregulated by the androgen receptor in skeletal muscle and regulates myoblast proliferation.
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In vivo expression of Odc1 is shown to be decreased >60% in muscle from male muscle-specific AR knockout mice, and androgens act via the AR to upregulate OdC1 in skeletal muscle myoblasts, and Odc 1 promotes myoblast proliferation and delays differentiation. Expand
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