C-terminal tail phosphorylation of N-formyl peptide receptor: differential recognition of two neutrophil chemoattractant receptors by monoclonal antibodies NFPR1 and NFPR2.

@article{Riesselman2007CterminalTP,
  title={C-terminal tail phosphorylation of N-formyl peptide receptor: differential recognition of two neutrophil chemoattractant receptors by monoclonal antibodies NFPR1 and NFPR2.},
  author={Marcia H Riesselman and Heini M Miettinen and Jeannie M. Gripentrog and Connie I. Lord and Brendan M Mumey and Edward A. Dratz and Jamal Stie and Ross M. Taylor and Algirdas J. Jesaitis},
  journal={Journal of immunology},
  year={2007},
  volume={179 4},
  pages={2520-31}
}
The N-formyl peptide receptor (FPR), a G protein-coupled receptor that binds proinflammatory chemoattractant peptides, serves as a model receptor for leukocyte chemotaxis. Recombinant histidine-tagged FPR (rHis-FPR) was purified in lysophosphatidyl glycerol (LPG) by Ni(2+)-NTA agarose chromatography to >95% purity with high yield. MALDI-TOF mass analysis (>36% sequence coverage) and immunoblotting confirmed the identity as FPR. The rHis-FPR served as an immunogen for the production of 2 mAbs… CONTINUE READING
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