C-terminal processing of barley alpha-amylase 1 in malt, aleurone protoplasts, and yeast.

@article{Sgaard1991CterminalPO,
  title={C-terminal processing of barley alpha-amylase 1 in malt, aleurone protoplasts, and yeast.},
  author={Morten S\ogaard and F. L. Olsen and Birte Svensson},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1991},
  volume={88 18},
  pages={8140-4}
}
C-terminal processing of low pI barley alpha-amylase (AMY1) results in multiple forms in malt, aleurone protoplasts, and transformed yeast. Expression of an AMY1 cDNA in yeast thus leads to four secreted forms with distinct pI values between 4.7 and 5.1 and essentially identical Mr. AMY1-1 and AMY1-2 lacking the C-terminal Arg-Ser are generated by carboxypeptidase in vitro from AMY1-3 and AMY1-4, respectively. In vivo processing is due to the KEX1-encoded yeast carboxypeptidase. AMY1-2 and AMY1… CONTINUE READING

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