C-terminal deletion mutant of pokeweed antiviral protein inhibits viral infection but does not depurinate host ribosomes.

@article{Tumer1997CterminalDM,
  title={C-terminal deletion mutant of pokeweed antiviral protein inhibits viral infection but does not depurinate host ribosomes.},
  author={Nilgun Ereken Tumer and Deborah J. Hwang and Maureen S. Bonness},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1997},
  volume={94 8},
  pages={3866-71}
}
Pokeweed antiviral protein (PAP), a 29-kDa protein isolated from Phytolacca americana, inhibits translation by catalytically removing a specific adenine residue from the large rRNA of the 60S subunit of eukaryotic ribosomes. In addition to its ribosome-inactivating ability, PAP has potent antiviral activity against many plant and animal viruses, including HIV. We recently described the isolation and characterization of nontoxic PAP mutants, NT123-2, which has a point mutation (E176V) in the… CONTINUE READING