C-SNARF-1 as a fluorescent probe for ph measurements in living cells: Two-wavelength-ratio method versus whole-spectral-resolution method

@inproceedings{Ribou2002CSNARF1AA,
  title={C-SNARF-1 as a fluorescent probe for ph measurements in living cells: Two-wavelength-ratio method versus whole-spectral-resolution method},
  author={Anne-C{\'e}cile Ribou and Jean Vigo and J Salmon},
  year={2002}
}
Measuring intracellular concentrations of ions (H+, Ca2+, Mg2+) is one of the challenges of modern cell biology. Fluorescence techniques can be used for this purpose since they are noninvasive, specific, and in general sensitive enough to require only low concentrations of the probes. In this paper, we describe two methods to measure pH by fluorescence spectroscopy and a way to extend the measurement to living cells. We use the fluorescent probe, C-SNARF-1, which exhibits a spectral shift of 45… CONTINUE READING