C‐terminal region‐dependent change of antibody‐binding to the Eighth Reelin repeat reflects the signaling activity of Reelin

@article{Kohno2009CterminalRC,
  title={C‐terminal region‐dependent change of antibody‐binding to the Eighth Reelin repeat reflects the signaling activity of Reelin},
  author={Takao Kohno and Yoshimi Nakano and N Kitoh and Hirokazu Yagi and Koichi Kato and Atsushi Baba and Mitsuharu Hattori},
  journal={Journal of Neuroscience Research},
  year={2009},
  volume={87}
}
Reelin is a secreted glycoprotein that plays pivotal roles in the development and function of the brain, but how it activates downstream intracellular signaling is not fully understood. We have recently reported that the highly conserved C‐terminal region (CTR) of Reelin is required for its full signaling activity, although the underlying mechanism remains unknown. During biochemical study of Reelin, we serendipitously found that one commercially available anti‐Reelin antibody G20 can bind to… 
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Cleavage within Reelin Repeat 3 Regulates the Duration and Range of the Signaling Activity of Reelin Protein*
TLDR
It is demonstrated that N-t cleavage of Reelin plays critical roles in regulating the duration and range of Regel functions both in the extracellular milieu and in the intracellular compartments.
Determination of cleavage site of Reelin between its sixth and seventh repeat and contribution of meprin metalloproteases to the cleavage.
TLDR
It is proposed that meprin α and mePRin β join the modulators of Reelin signalling as they cleave Reelin at a specific site and are upregulated under specific pathological conditions.
Functional Importance of Covalent Homodimer of Reelin Protein Linked via Its Central Region*
TLDR
The results of this study indicate that an intact higher order architecture of reelin multimer maintained by both Cys2101-mediated homodimerization and other non-covalent association present elsewhere in the reelin primary structure are essential for exerting its full biological activity.
Importance of Reelin C-Terminal Region in the Development and Maintenance of the Postnatal Cerebral Cortex and Its Regulation by Specific Proteolysis
TLDR
Results from ectopic expression of mutant Reelin proteins in utero suggest that the dendrite development and maintenance of the MZ require Reelin protein with an intact CTR, indicating that the CTR is necessary for efficient induction of Dab1 phosphorylation in vivo.
Structural Insights into Reelin Function: Present and Future
TLDR
Current findings on the structure of Reelin and its binding to the ApoER2 and VLDLR receptors are discussed, and some areas where proteomics and structural biology can help understanding Reelin function in brain development and human health are discussed.
Regulated Proteolytic Processing of Reelin through Interplay of Tissue Plasminogen Activator (tPA), ADAMTS-4, ADAMTS-5, and Their Modulators
TLDR
Serine protease tissue plasminogen activator and two matrix metalloproteinases are identified and it is demonstrated that in the murine hippocampus, the expression levels and localization of Reelin proteases largely overlap with that of Reangel, and changes in their protein levels coincided with accelerated Reelin aggregation in a mouse model of AD.
The functions of Reelin in membrane trafficking and cytoskeletal dynamics: implications for neuronal migration, polarization and differentiation.
TLDR
This review addresses and discusses in detail the participation of reelin in the processes underlying neurogenesis, neuronal migration in the cerebral cortex and the hippocampus; and the polarization, differentiation and maturation processes that neurons experiment in order to be functional in the adult brain.
C-Terminal Region Truncation of RELN Disrupts an Interaction with VLDLR, Causing Abnormal Development of the Cerebral Cortex and Hippocampus
TLDR
A new Reln mutant with a truncation of the C-terminal region (CTR) domain shows that Reln mutation can cause abnormal phenotypes in the cortex and hippocampus without cerebellar hypoplasia, and suggests thatreln mutations may cause more subtle forms of human brain malformation than classic lissencephalies.
Ceruloplasmin-induced aggregation of P19 neurons involves a serine protease activity and is accompanied by reelin cleavage
Aberrant fragment of Dab1 protein is present in yotari mouse
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References

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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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