Identification of Beta-2 as a Key Cell Adhesion Molecule in PCa Cell Neurotropic Behavior: A Novel Ex Vivo and Biophysical Approach
BACKGROUND Bystin, Trophinin, and Tastin are component proteins of an adhesion molecule complex that plays a crucial role in the initial attachment of the embryo to the uterus. METHODS Profiling of genes differentially expressed in the perineural invasion (PNI) in vitro model by gene microarray analysis showed overexpression of bystin in prostate cancer cells co-cultured with nerves. Validation was performed at the RNA levels using quantitative PCR. RESULTS Bystin is overexpressed in cells co-cultured with nerves. Bystin is also present in human prostatic carcinoma (PCa) cells in PNI location in increasing gradient. Bystin is present in the supernatant of the PNI co-culture. CONCLUSIONS Their adhesive and invasive functions in the trophoblast suggest that they might also play a role in perineural adhesion. Bystin is, therefore, an important therapeutic target for neurotropic cancers.