Bxz1, a new generalized transducing phage for mycobacteria.

  title={Bxz1, a new generalized transducing phage for mycobacteria.},
  author={Sunhee Lee and Jordan Kriakov and Catherine Vilch{\'e}ze and Zhiyan Dai and Graham F. Hatfull and William Robert Jacobs},
  journal={FEMS microbiology letters},
  volume={241 2},
We have isolated and characterized a new generalized transducing phage, Bxz1, from soil sampling at a neighboring Wildlife Preservation Park. The hosts of the phage, measured by the formation of plaques, include fast growing Mycobacterium smegmatis and Mycobacterium vaccae. Bxz1 is capable of transducing chromosomal markers, point mutations, and plasmids at frequencies ranging from 10(-8) to 10(-6) per plaque forming unit between strains of M. smegmatis. We also demonstrated cotransduction of a… CONTINUE READING

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