Buthionine sulfoximine (BSO)-mediated improvement in cultured embryo quality in vitro entails changes in ascorbate metabolism, meristem development and embryo maturation

@article{Stasolla2008ButhionineS,
  title={Buthionine sulfoximine (BSO)-mediated improvement in cultured embryo quality in vitro entails changes in ascorbate metabolism, meristem development and embryo maturation},
  author={Claudio Stasolla and Mark F Belmonte and Muhammad Sufyan Tahir and Mohamed Abdelsamad Elhiti and Khalil Khamiss and Ronny Joosen and Chris Maliepaard and Gerald Pattenden and Branimir Gjetvaj and Kim Boutilier},
  journal={Planta},
  year={2008},
  volume={228},
  pages={255-272}
}
Applications of buthionine sulfoximine (BSO), an inhibitor of GSH (reduced glutathione), which switches the cellular glutathione pool towards the oxidized form GSSG, positively influences embryo quality by improving the structure of the shoot apical meristem and promoting embryo maturation, both of which improve the post-embryonic performance of the embryos. To investigate the mechanisms underlying BSO-mediated improvement in embryo quality the transcript profiles of developing Brassica napus… CONTINUE READING
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