Butanol Isomers Exert Distinct Effects on Voltage-Gated Calcium Channel Currents and Thus Catecholamine Secretion in Adrenal Chromaffin Cells

  title={Butanol Isomers Exert Distinct Effects on Voltage-Gated Calcium Channel Currents and Thus Catecholamine Secretion in Adrenal Chromaffin Cells},
  author={S. McDavid and M. B. Bauer and Rebecca L. Brindley and M. Jewell and K. Currie},
  journal={PLoS ONE},
Butanol (C4H10OH) has been used both to dissect the molecular targets of alcohols/general anesthetics and to implicate phospholipase D (PLD) signaling in a variety of cellular functions including neurotransmitter and hormone exocytosis. Like other primary alcohols, 1-butanol is a substrate for PLD and thereby disrupts formation of the intracellular signaling lipid phosphatidic acid. Because secondary and tertiary butanols do not undergo this transphosphatidylation, they have been used as… Expand
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  • J. Coorssen
  • Biology, Medicine
  • The American journal of physiology
  • 1996
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