Bulk isolation of renal PCT and PST. II. Differential responses to anoxia or hypoxia.

@article{Ruegg1990BulkIO,
  title={Bulk isolation of renal PCT and PST. II. Differential responses to anoxia or hypoxia.},
  author={Charles E. Ruegg and Lazaro J. Mandel},
  journal={The American journal of physiology},
  year={1990},
  volume={259 1 Pt 2},
  pages={F176-85}
}
Innate biochemical responses of rabbit renal proximal convoluted (PCT) and straight (PST) segments following in vitro exposure to anoxia or hypoxia were investigated to delineate the mechanisms responsible for segment-selective injury in vivo. After bulk isolation, suspensions (1 mg/ml) enriched in either PCT or PST were preincubated in Dulbecco's modified Eagle's-Ham's F-12 medium for 1 h before being exposed to either 40 min of anoxia (N2) or 120 min of hypoxia (1% O2) and 1 h of recovery… CONTINUE READING

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