Building with cells

  title={Building with cells},
  author={Joel Voldman},
  journal={Nature Materials},
  • J. Voldman
  • Published 1 July 2003
  • Biology
  • Nature Materials
New ways to interact with biological cells in vitro offer greater levels of control over their location and milieu — much as they would experience in real tissues in vivo. Such microscale control reveals new insights into their biology and may lead to new technologies. 
Intracellular silicon chips in living cells.
This paper aims to demonstrate the efforts towards in-situ applicability of EMMARM, as to provide real-time information about concrete mechanical properties such as E-modulus and compressive strength.
Biomechanical Approaches for Studying Integration of Tissue Structure and Function in Mammary Epithelia
It is argued that the combination of biomechanical tools with current efforts in mathematical modeling and in cell and molecular biology applied to 3D cultures provides a powerful approach to unravel the complexity of tissue-specific structure-function relationships.
Intracellular polysilicon barcodes for cell tracking.
The utility of the barcodes for cell tracking is demonstrated by following individual cells for up to ten days in culture and recording their locomotion.
Evolution of Biochip Technology: A Review from Lab-on-a-Chip to Organ-on-a-Chip
Applications of LOC devices as a versatile tool for POC applications are focused on, which include current progress in OOC platforms towards body-on-a-chip, and concluding remarks and future perspectives are provided.
High-Sensitivity in Dielectrophoresis Separations
Efforts to leverage that most compelling aspect of DEP—an actuation force that depends on particle electrical properties—in the background of phenotypic variations in cell size are explored.
Microsystems for controlled genetic perturbation of live Drosophila embryos: RNA interference, development robustness and drug screening
A bio-compatible microfluidic device that automatically positions live embryos within a fluid-filled imaging channel and exposes embryos to constant or biphasic temperature or different drug concentrations is designed and it is demonstrated that a thermal gradient applied across live Drosophila embryos results in asynchronous cellularization.
Micropatterned polyelectrolyte nanofilms promote alignment and myogenic differentiation of C2C12 cells in standard growth media
The results validate PEM microstructured devices, to a greater extent for (PAH/PSS)5‐coated microgrooves, as biocompatible and innovative tools for tissue engineering applications and molecular dissection of events controlling C2C12 skeletal muscle regeneration without switching to their optimal differentiative culture media in vitro.
The Patterning and Alignment of Muscle Cells Using the Selective Adhesion of Poly(oligoethylene glycol methyl ether methacrylate)‐based ABA Block Copolymers
The selective adhesion/delamination behavior of poly(oligoethylene glycol methyl ether methacrylate)-based ABA block copolymers is exploited to generate patterned substrates consisting of alternating lanes of glass and copolymer-coated gold, forming a promising 2D in-vitro model for muscles.


Probing heterotypic cell interactions: hepatocyte function in microfabricated co-cultures.
Heterotypic cell interactions in co-cultures of hepatocytes and 3T3 are probed in order to better understand the cellular microenvironment necessary to induce and stabilize liver-specific functions and suggested that heterotypiccell contact is necessary for induction of these functions.
Laminar flows: Subcellular positioning of small molecules
The use of multiple laminar streams in a microfluidic channel to deliver membrane-permeable molecules to selected subcellular microdomains opens up avenues for non-invasively visualizing, probing and manipulating the cellular metabolic and structural machinery.
Geometric control of cell life and death.
Human and bovine capillary endothelial cells were switched from growth to apoptosis by using micropatterned substrates that contained extracellular matrix-coated adhesive islands of decreasing size