Building epithelial architecture: insights from three-dimensional culture models

@article{OBrien2002BuildingEA,
  title={Building epithelial architecture: insights from three-dimensional culture models},
  author={Lucy Erin O’Brien and Mirjam M Zegers and Keith E. Mostov},
  journal={Nature Reviews Molecular Cell Biology},
  year={2002},
  volume={3},
  pages={531-537}
}
How do individual cells organize into multicellular tissues? Here, we propose that the morphogenetic behaviour of epithelial cells is guided by two distinct elements: an intrinsic differentiation programme that drives formation of a lumen-enclosing monolayer, and a growth factor-induced, transient de-differentiation that allows this monolayer to be remodelled. 
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This unit discusses technical aspects for culturing and imaging MDCK 3-D culture for both fixed3-D cultures and live-cell imaging to study epithelial architecture and morphogenesis under close-to-physiological conditions.
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