Building blocks.

  title={Building blocks.},
  author={Lutz Hilterhaus and Andreas Liese},
  journal={Advances in biochemical engineering/biotechnology},
This contribution illustrates the versatility of fundamental approaches in industrial biotransformations. The applicability of biotechnology in organic synthesis on an industrial scale is discussed, followed by an overview of historical development and future progress. This chapter depicts three different approaches for the use of biocatalysts in production processes: non-chiral synthesis, asymmetric synthesis, and racemic and dynamic resolution. Applications for whole cells and isolated… Expand
Employing modular polyketide synthase ketoreductases as biocatalysts in the preparative chemoenzymatic syntheses of diketide chiral building blocks.
These syntheses demonstrate the ability of PKS enzymes to economically and greenly generate diverse chiral building blocks on a preparative scale. Expand
A chemo-enzymatic route to synthesize (S)-γ-valerolactone from levulinic acid
The conversion of keto ester to ethyl levulinate was successfully reduced by (S)-specific carbonyl reductase from Candida parapsilosis (CPCR2) in a substrate-coupled cofactor regeneration system utilizing isopropanol as cosubstrate. Expand
Dissolving carbon dioxide in high viscous substrates to accelerate biocatalytic reactions.
By dissolving high dense carbon dioxide into the reaction system the viscosity could be reduced, keeping the temperature at an acceptable level at the same time and the reaction rate was increased by a factor of 4. Expand
Whole cell microbial transformation in cloud point system
The basic properties of cloud point system including phase separation and solubilization are introduced and examples of whole cell microbial transformation in cloud point systems, especially in situ extraction of moderate polar substrate/product are presented. Expand