Corpus ID: 46206943

Buffered phenol formaldehyde (pH 7.0 and pH 5.5): improved fixation in an enclosed tissue processor.

@article{Slidders1989BufferedPF,
  title={Buffered phenol formaldehyde (pH 7.0 and pH 5.5): improved fixation in an enclosed tissue processor.},
  author={W. Slidders and D. Hopwood},
  journal={Medical laboratory sciences},
  year={1989},
  volume={46 1},
  pages={
          74-6
        }
}
  • W. Slidders, D. Hopwood
  • Published 1989
  • Chemistry, Medicine
  • Medical laboratory sciences
  • The addition of 2% phenol has a marked adjuvant effect on 4% formaldehyde as a fixative. Sequential use of phenol formaldehyde buffered to pH 7.0 and pH 5.5 results in improved fixation in material rapidly advanced to paraffin in an enclosed tissue processor. Sections stained with alum haematoxylin and eosin show improved nuclear and cytoplasmic detail, reduced tissue shrinkage and an absence of formalin pigment. Excellent results are obtained with conventional special stains and… CONTINUE READING
    10 Citations
    Tissue fixation with phenol—formaldehyde for routine histopathology
    • 11
    Histology without formalin?
    • 81
    Use of benzidine for histological demonstration of haemoglobin in human bite marks.
    • 4
    • PDF
    Effects of treatment on the histopathology of leprosy.
    • 5
    • PDF