Buckwheat trypsin inhibitor enters Hep G2 cells by clathrin-dependent endocytosis.


Recombinant buckwheat trypsin inhibitor (rBTI) was studied to evaluate if it could enter cancer cells and to determine the mechanism. Fluorescein isothiocyanate-labelled buckwheat trypsin inhibitor (FITC-BTI) entered Hep G2 cells in a concentration-dependent manner. FITC-BTI colocalised with labelled transferrin (Tf) in the punctate structure, implying that… (More)
DOI: 10.1016/j.foodchem.2013.04.001


7 Figures and Tables