Bubble free cell culture aeration with porous moving membranes.

  title={Bubble free cell culture aeration with porous moving membranes.},
  author={Juergen Lehmann and G W Piehl and Rainer Schulz},
  journal={Developments in biological standardization},
For the cultivation of mammalian cells a new aeration system was developed. Its construction with hydrophobic porous Accurel hollow fiber membranes and the use in reactors with 1 and 20 l reaction volume will be presented and introduced by means of the cultivation of a genetically manipulated mouse cell line. The mass transfer capacity of this system is demonstrated as a function of gas flow rates through the membrane and of the membrane movement. 

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