Bubble dynamics in double-stranded DNA.

@article{AltanBonnet2003BubbleDI,
  title={Bubble dynamics in double-stranded DNA.},
  author={G. Altan-Bonnet and A. Libchaber and O. Krichevsky},
  journal={Physical review letters},
  year={2003},
  volume={90 13},
  pages={
          138101
        }
}
We report the first measurement of the dynamics of bubble formation in double-stranded DNA. Fluctuations of fluorescence of a synthetic DNA construct, internally tagged with a fluorophore and a quencher, are monitored by fluorescence correlation spectroscopy. The relaxation dynamics follow a multistate relaxation kinetics, with a characteristic time scale of 50 microseconds. A simple model of bubble dynamics based on constant zipping-unzipping rates is proposed to account for our experimental… Expand
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