Brush-border amino acid transport mechanisms in carnivorous eel intestine.

  title={Brush-border amino acid transport mechanisms in carnivorous eel intestine.},
  author={Carlo Storelli and Sebastiano Vilella and Maria Pia Romano and Michele Maffia and Giuseppe Cassano},
  journal={The American journal of physiology},
  volume={257 3 Pt 2},
Brush-border membrane vesicles (BBMV) were prepared from European eel (Anguilla anguilla) intestinal epithelium by a magnesium-ethylene glycolbis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) precipitation technique. Amino acid transport by these purified vesicle preparations was investigated using either radiolabeled substrates or the voltage-sensitive fluorescent dye 3,3'-diethylthiadicarbocyanine iodide [DiSC2(5)]. All amino acids tested exhibited carrier-mediated, Na+-dependent… CONTINUE READING