Brugia malayi and B. pahangi: cultivation in vitro of infective larvae to the fourth and fifth stages.

@article{Mak1983BrugiaMA,
  title={Brugia malayi and B. pahangi: cultivation in vitro of infective larvae to the fourth and fifth stages.},
  author={Joon Wah Mak and P. K. Lim and Betty Kim Lee Sim and L M Liew},
  journal={Experimental parasitology},
  year={1983},
  volume={55 2},
  pages={243-8}
}
Cultivation of fourth stage Brugia pahangi and B. malayi larvae from infective larvae (stage 3) were obtained in culture medium RPMI 1640 supplemented with 10% human AB serum and an LCC-MK2 rhesus monkey kidney continuous cell line feeder layer. This culture system kept larvae alive in excess of 7 weeks, and served as a source for collection of the worms' secretory, excretory, and moulting antigens. 

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