Brown-adipose-tissue mitochondria: the regulation of the 32000-Mr uncoupling protein by fatty acids and purine nucleotides.

@article{Rial1983BrownadiposetissueMT,
  title={Brown-adipose-tissue mitochondria: the regulation of the 32000-Mr uncoupling protein by fatty acids and purine nucleotides.},
  author={Eduardo Rial and A J Poustie and Dr M. Gary Nicholls},
  journal={European journal of biochemistry},
  year={1983},
  volume={137 1-2},
  pages={197-203}
}
The increased proton permeability induced by the addition of a synthetic proton translocator to non-respiring hamster brown-fat mitochondria is unaffected by purine nucleotide addition. In contrast the permeability induced by fatty acids is inhibited by nucleotide, indicating that fatty acids act at the 32000-Mr uncoupling protein. Fatty acids lower the affinity of nucleotide binding to the 32000-Mr protein, but not sufficiently to explain their uncoupling action. The sensitivity of the fatty… CONTINUE READING