Bromelain: an overview of industrial application and purification strategies

@article{Arshad2014BromelainAO,
  title={Bromelain: an overview of industrial application and purification strategies},
  author={Z. Arshad and A. Amid and F. Yusof and I. Jaswir and K. Ahmad and Show Pau Loke},
  journal={Applied Microbiology and Biotechnology},
  year={2014},
  volume={98},
  pages={7283-7297}
}
This review highlights the use of bromelain in various applications with up-to-date literature on the purification of bromelain from pineapple fruit and waste such as peel, core, crown, and leaves. Bromelain, a cysteine protease, has been exploited commercially in many applications in the food, beverage, tenderization, cosmetic, pharmaceutical, and textile industries. Researchers worldwide have been directing their interest to purification strategies by applying conventional and modern… Expand
BROMELAIN SEPARATION AND PURIFICATION PROCESSES FROM PINEAPPLE EXTRACT
The concern about the effective use of agro-industrial residues from the food industry has increased. Bromelain is an enzyme mainly obtained from the pineapple plant (A. comosus), including its partsExpand
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An up-to-date compilation of data regarding the commercialisation of bromelain in the clinical, pharmaceutical and industrial fields is provided in this review. Expand
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This paper aims to review the available literature for the use of nanoparticles and bromelain in the pharmaceutical nanotechnology field and suggest ways to enhance active's stability, solubility and permeability, and decrease toxicity. Expand
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Bromelain is a concoction of sulfhydryl proteolytic enzymes. Depending upon the site of extraction it can be regarded as either stem bromelain (SBM) (EC 3.4.22.32) or fruit bromelain (FBM) (ECExpand
Application of membrane-based technology for purification of bromelain
About 60% of world’s commercial enzyme products are proteases, giving promising opportunity to derive such enzymes sustainably from waste sources. Bromelain is a crude protease occurring naturally inExpand
Optimization of bromelain isolation from pineapple byproducts by polysaccharide complex formation
Abstract A simple method for bromelain extraction from industrial pineapple residues (stems and peels) was developed and optimized through factorial experimental design. The developed methodology,Expand
Single step purification of bromelain from Ananas comosus pulp using a polymer/salt aqueous biphasic system
Abstract Bromelain is an important industrial proteolytic enzyme due to the wide application in food, cosmetic and therapeutic industries. There is a great demand in developing a cost-efficientExpand
Preparation of dendritic polymer-based magnetic carrier for application of bromelain separation and purification.
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A novel magnetic carrier with high stability and fabulous performance for bromelain separation and purification in solution is presented and the practical application that the magnetic carrier can efficiently separate bromELain from the crude extract of pineapple peel is achieved. Expand
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References

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Purification of Bromelain from Ananas Comosus by Peg/phosphate Atps
The bromelains are proteases from vegetable origin, obtained from several species of Bromeliaceae family, which are found in pineapple’s stem and fruit. The bromelains uses are based on itsExpand
Simple Purification of Bromelain from Pineapple
Bromelain(EC 3.4.22.4), the collective name for the proteolytic enzymes found in tissues of the plant family Bromeliaceae(pineapple), has been used as a tenderizing agent in food processing, and asExpand
Simple Purification of Bromelain from Pineapple
Bromelain(EC 3.4.22.4), the collective name for the proteolytic enzymes found in tissues of the plant family Bromeliaceae(pineapple), has been used as a tenderizing agent in food processing, and asExpand
Native and Biotechnologically Engineered Plant Proteases with Industrial Applications
TLDR
The present review focuses on the applications of traditional plant proteases as well as new proteases discovered during the last 20 years, some of which have already been genetically engineered either to increase production or to strengthen some of their physicochemical properties. Expand
Purification of bromelain from pineapple wastes by ethanol precipitation
Abstract Bromelain is an aqueous extract of pineapple that contains a complex mixture of proteases and non-protease components. These enzymes perform an important role in proteolytic modulation ofExpand
Extraction of bromelain from pineapple core and purification by RME and precipitation methods
Demand for bromelain is increasing because of its application in various fields like medical, food and cosmetics. While bromelain extracted from pineapple stem is commercially known, presence ofExpand
Bromelain purification through unconventional aqueous two-phase system (PEG/ammonium sulphate)
TLDR
The analysis of the results revealed that the use of the desirability optimisation methodology (DOM) was necessary to achieve higher purification factor values and greater bromelain recovery. Expand
Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus)
Bromelain is a complex of proteolytic enzymes obtained from pineapple (Ananas comosus) fruits and stem. These proteolytic enzymes are tools that promise an impressive number of medical andExpand
Comparative study of extraction, purification and estimation of bromelain from stem and fruit of pineapple plant.
Bromelain is a major proteinase, isolated from pineapple (Ananas comosus). In the plant, bromelain is accumulated in the entire plant to different extent and properties depending on its source. TheExpand
Pineapple wastes: A potential source for bromelain extraction
Abstract This study investigates the isolation and characterization of bromelain extract from the wastes of Nang Lae and Phu Lae pineapple cultivars (economical fruits of Chiang Rai province,Expand
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