Broad specificity indirect competitive immunoassay for determination of nitrofurans in animal feeds.

@article{Li2010BroadSI,
  title={Broad specificity indirect competitive immunoassay for determination of nitrofurans in animal feeds.},
  author={Jun Li and Jing Liu and Hui-Cai Zhang and Hui Li and Jian-ping Wang},
  journal={Analytica chimica acta},
  year={2010},
  volume={678 1},
  pages={
          1-6
        }
}
  • Jun Li, Jing Liu, +2 authors Jian-ping Wang
  • Published 2010
  • Chemistry, Medicine
  • Analytica chimica acta
  • A generic hapten of nitrofurans was synthesized by derivatization of 5-nitrofurfural with diamine, and the hapten was coupled to carrier protein to prepare different immunogens and coating antigens by using diazotization method and glutaraldehyde method. The obtained novel polyclonal antibodies from immunized rabbits showed broad cross reactivity among seven nitrofurans. After assessment of four coating antigen/antibody combinations, an indirect competitive immunoassay was developed to… CONTINUE READING

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